rabbit polyclonal antibody against hsp60  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: TFAM expression in brown adipocytes confers obesity resistance by secreting extracellular vesicles that promote self-activation

doi: 10.1016/j.isci.2022.104889

Figure Lengend Snippet: Effects of hTFAM overexpression on white and brown adipose tissue phenotypes (A) Verification of hTFAM protein expression in epididymal (e)- and inguinal (i)-WAT and BAT. (B) The h- and m- TFAM protein levels were evaluated using western blot analysis. Pictures represent western blots for h- and m- TFAM in BAT. (C) The ratio between hTFAM and mTFAM protein expression in Tg and TgTg BAT. (D) The h- and m- TFAM protein levels were normalized by the levels of HSP60. The quantitative results are expressed as the mean percentage of the level in the Tg ± standard error (SE) (n = 3/group). Total protein extracts from e− and i-WAT and BAT probed with a polyclonal antiserum against h- and m- TFAM. (E) Photographs show representative conditions of e − and i-WAT, liver, and BAT from NCD-fed WT, Tg, and TgTg mice. (F) Effect of hTFAM overexpression on e-adipocyte size. Pictures are representative of each sample stained with hematoxylin and eosin and examined by optical microscopy. Scale bar represents 100 μm. (G) Frequency distribution of the e-adipocyte area in NCD-fed WT, Tg, and TgTg mice. ∗∗p < 0.01, versus Tg mice. Two-tailed unpaired Student’s t test in (C) and (D).

Article Snippet: Rabbit polyclonal anti HSP60 (D307) , Cell Signaling Technology , Cat# 4870; RRID: AB_2295614.

Techniques: Over Expression, Expressing, Western Blot, Staining, Microscopy, Two Tailed Test

Journal: iScience

Article Title: TFAM expression in brown adipocytes confers obesity resistance by secreting extracellular vesicles that promote self-activation

doi: 10.1016/j.isci.2022.104889

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti HSP60 (D307) , Cell Signaling Technology , Cat# 4870; RRID: AB_2295614.

Techniques: Staining, Recombinant, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Membrane, Chromatography, Transmission Assay, Microscopy

Journal: FASEB bioAdvances

Article Title: Interplay in neural functions of cell adhesion molecule close homolog of L1 (CHL1) and Programmed Cell Death 6 (PDCD6).

doi: 10.1096/fba.2021-00027

Figure Lengend Snippet: FIGURE 1 Expression of CHL1 and PDCD6 in whole brain and cerebellum during development. Whole brains and cerebella from age-matched wild-type (+/+) and CHL1-deficient (−/−) littermates at three ages (3 days: 3D, 1 month: 1 M, and 6 months: 6 M) were used. The whole brains (A) and cerebella (D) were dissected, homogenized, and analyzed for the expression of CHL1, PDCD6, and HSP60 (loading control) by western blot analysis. (B, C, E, F) Quantification of the relative expression levels of CHL1 (B, E) and PDCD6 (C, F) normalized to HSP60. Data show average values ± SEM. For the analysis, three brains or cerebella per genotype and age were used

Article Snippet: Rabbit polyclonal HSP60 antibody (#4870S) was purchased from Cell Signaling Technology.

Techniques: Expressing, Control, Western Blot